Methods SH was applied in the short-term and middle-term cornea preservation. The preservation effect was evaluated by corneal morphology, trypan blue-alizarin red staining, activity assay of SDH and G-6-P enzymes, and ultrastructural observation of corneal endothelium. 方法分别在短期和中期保存中应用SH保存角膜,对保存不同时间的角膜行大体形态,活体内皮镜,苔盼兰-茜素红染色,SDH、G-6-P酶检测和电镜观察。
At 6 hours postoperatively, trypan blue-alizarin red active staining was performed, and the changes of slight structures of corneal endothelium were observed under light microscope. 术后6h应用锥蓝-茜素红活性染色,光镜下观察各组角膜内皮细胞微细结构的变化。
Spectrophotometric determination of amikacin sulfate with tiger red and trypan red 虎红、曲利本红-阿米卡星的光度法研究
Methods Mix-cultured conical neurons of SD rats were subjected to mechanical scratch injury, and the degree of injury were assessed by cell count with trypan blue staining and by lactate dehydrogenase ( LDH) test. The intact cells were confirmed with neutral red counter staining. 方法采用机械划痕创伤性损伤培养大鼠皮层神经元,根据培养细胞锥虫篮活力计数和培养上清乳酸脱氢酶(LDH)检测评估损伤程度,用中性红复染确定未损伤细胞。
In acid solution, amikacin sulfate ( AMK) react with tiger red ( TIR) or trypan red ( TRR) to form an ion-association complex. 在酸性条件下,硫酸阿米卡星(AMK)与虎红(TIR)、曲利本红(TRR)均可反应生成离子缔合物。
Conclusion It is the same effect to detect the activity of the cells between trypan blue and neutral red. 结论锥虫蓝和中性红染色,都能较好地检测细胞的活性。
Study on the spectrophotometric determination of aminoglycoside antibiotics with trypan red 曲利本红分光光度法测定氨基糖苷类抗生素
Staining the separated cells by trypan blue and neutral red. 锥虫蓝和中性红染色检测分离细胞的活性。
Isolation, purification, culture and identification of rat Sertoli cells in vitro: Take 18 to 21 day-old male SD rat testis, culture primary Sertoli cells in vitro, identify cell viability by trypan blue, and identify cells purity by oil red O.2. 方法1.体外分离、纯化、培养和鉴定大鼠睾丸支持细胞:取18~21日龄雄性SD大鼠的睾丸组织,体外原代培养睾丸支持细胞,苔盼蓝鉴定细胞活力,油红O鉴定细胞纯度。
The differences of the viability of corneal endothelial cells after different preservation methods were compared dyeing with trypan blue and alizarin red. 2. 采用台盼蓝-茜素红染色对不同保存方法的角膜内皮细胞进行评价。